Goat anti Mouse IgG2a FITC

Name

Goat anti Mouse IgG2a FITC

Size

1 ml

Catalog number

43R-IG063ft

Price

451 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Raised in

Goat

Specificity

Murine

Cross Reactivity

Should be determined by the end user

Isotypes

IgG

Conjugate/Tag

FITC

Method of Purification

Goat anti Mouse IgG2a (FITC) was purified by affinity chromatography.

Concentration

1.0 mg/ml

Form & Buffer

PBS, 0.2% BSA, and 0.1% NaN3.

Storage

Store at 4 deg C.

Shipping conditions

Blue Ice

Tested for

FC; ICC; IHC

Usage Recommendations

ICC: 1:200-1:2,000, IHC: 1:200-1:2,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

Properties

This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.

Conjugation

Anti-FITC Antibody

Latin name

Capra aegagrus hircus,Mus musculus

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.