Goat anti Mouse IgG + IgM rhodamine

Name

Goat anti Mouse IgG + IgM rhodamine

Size

1 mg

Catalog number

43R-1389

Price

204 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Mouse IgG + IgM

Raised in

Goat

Specificity

This antibody reacts with heavy (gamma) chains on mouse IgG and heavy (mu) chains on mouse IgM and light chains on all mouse immunoglobulins.

Cross Reactivity

No reactivity is observed to non-immunoglobulin mouse serum proteins.

Conjugate/Tag

Rhodamine

Grade & Purity

Grater than 95% pure

Method of Purification

Affinity purified using solid phase Mouse IgG + IgM

Source

Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Concentration

1.0 mg/ml (lot specific)

Form & Buffer

Lyophilized from 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2. Dilute in dH2O to a concentration of 1 mg/ml

Storage

Store at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

FC; IF; IHC

Usage Recommendations

The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

Latin name

Capra aegagrus hircus,Mus musculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG,IgM

Properties

Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.