Goat anti Mouse IgG + IgM H + L Alk Phos

Name

Goat anti Mouse IgG + IgM H + L Alk Phos

Size

500 µg

Catalog number

43C-CB5175

Price

414 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Goat anti-mouse IgG/IgM (H+L) (Alk Phos) was raised in goat using murine IgG and IgM whole molecules ..

Raised in

Goat

Specificity

Murine IgG/IgM

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

Alkaline Phosphatase

Method of Purification

Goat anti Mouse IgG + IgM (H + L) (Alk Phos) was purified by affinity chromatography.

Concentration

1.0 mg/ml

Form & Buffer

0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.

Storage

Store at 4 deg C. DO NOT FREEZE.

Shipping conditions

Blue Ice

Tested for

ELISA; IHC; WB

Usage Recommendations

ELISA: 1:4,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

Latin name

Capra aegagrus hircus,Mus musculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG,IgM

Properties

Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.