GFP antibody biotin
1 mg
60R-GG001bt
833 EUR
Primary Antibody
Conjugated Polyclonal Antibodies
Miscellaneous
GFP antibody (biotin) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria ..
Goat
Jellyfish GFP
Should be determined by the end user
Biotin
GFP antibody (biotin) was purified by affinity chromatography.
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Blue Ice
ELISA; IHC; WB
ELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
anticorps