Donkey anti Goat IgG H + L biotin

Name

Donkey anti Goat IgG H + L biotin

Size

500 µl

Catalog number

43R-ID004bt

Price

290 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Donkey anti-goat IgG (H + L) (biotin) was raised in donkey using goat IgG (H & L) ..

Raised in

Donkey

Specificity

Goat

Cross Reactivity

Should be determined by the end user

Conjugate/Tag

Biotin

Method of Purification

Donkey anti Goat IgG (H + L) (biotin) was purified by affinity chromatography.

Form & Buffer

Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.

Storage

Store at 4 deg C.

Shipping conditions

Blue Ice

Tested for

ELISA; FC; ICC; IHC; WB

Usage Recommendations

ELISA: 1:20,000-1:400,000, FC: 1:200-1:1,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, WB: 1:20,000-1:400,000

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Latin name

Equus africanus asinus,Capra aegagrus hircus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Additional isotype

IgG