Biotin antibody
1 mg
70C-CR7026GAP
488 EUR
Primary Antibody
Polyclonal Antibodies, Purified
Miscellaneous
Biotin antibodies were raised in goat using biotin conjugated to KLH ..
Goat
Should be determined by the end user
Biotin antibody was purified by Ion exchange chromatography followed by dialysis.
1.0 mg/ml
0.02M K3PO4,pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Blue Ice
ELISA; IHC; WB
ELISA: 1:20,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
anticorps