Goat anti Rat IgG H + L biotin

Name

Goat anti Rat IgG H + L biotin

Size

2 mg

Catalog number

43R-1534

Price

327 EUR

More details

Category

Secondary Antibody

Antibody Subtype

Conjugated Secondary Antibodies

Area of research

Immunology

Type of Immunogen

Purified Rat IgG (H&L)

Raised in

Goat

Specificity

This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.

Cross Reactivity

No reactivity is observed to non-immunoglobulin mouse serum proteins or IgG from human or mouse.

Conjugate/Tag

Biotin

Grade & Purity

Grater than 95% pure

Method of Purification

Affinity purified using solid phase Rat IgG (H&L)

Source

Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Concentration

1.0 mg/ml (lot specific)

Form & Buffer

Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.

Storage

Store at 2-8 deg C.

Shipping conditions

Blue Ice

Tested for

Please refer to the datasheet

Usage Recommendations

1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Conjugation

Biotinylated

Latin name

Capra aegagrus hircus,Rattus norvegicus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Additional isotype

IgG