Goat anti-Rabbit IgG (H&L), F(ab)'2 Fragment - Affinity Pure, Biotin Conjugate, min x w/ bovine, human, or mouse IgG or serum proteins[IgG (H&L), F(ab)'2 Fragment]

Name

Goat anti-Rabbit IgG (H&L), F(ab)'2 Fragment - Affinity Pure, Biotin Conjugate, min x w/ bovine, human, or mouse IgG or serum proteins[IgG (H&L), F(ab)'2 Fragment]

Size

NA

Catalog number

MBS687640

Price

5 EUR

More details

Products_type

Secondary Antibody

Reactivity

Rabbit.Cross Reactivity: Based on IEP, no reactivity is observed to:non-immunoglobulin rabbit serum proteinsserum proteins from bovine, human, or mouseIgG from human or mouse

Tissue

serum

Test

A high affinity purification column was use to purify Goat anti-Rabbit IgG (H&L) F(ab)'2 - Pure, Conjugate, min x w/ , mouse IgG serum proteins[IgG (H&L) F(ab)'2 ] by MyBioSource by chromatographic size exclusion.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The ultrapure Goat anti-Rabbit IgG (H&L) F(ab)'2 - Pure, Conjugate, min x w/ , mouse IgG serum proteins[IgG (H&L) F(ab)'2 ] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Conjugation

Biotinylated

Additional isotype

Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.Heavy & Light chains,IgG

Latin name

Capra aegagrus hircus,Mus musculus,Oryctolagus cuniculus

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Serum should be stored at +5°C

Group

Sera